Promoter
EF1a.
Reporter
Firefly Luc.
Selection
Blasticidin.
Species
Human/Mouse.
Serotype
VSV-G.
Application
Monitor transcriptional activity of T-bet in mammalian cells, screen for activators or inhibitors of T-bet transcriptional activity. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc. The T-bet Reporter Lentivirus is constructed with tandem repeats of the consensus T-box transcription factor expressed in T-cells (T-bet or TBX21) response elements which is transcriptionally responsive to the transcription factor T-bet. T-bet is primarily known for its role in driving Th1 T cell lineage commitment. T-bet is also involved in immunoglobulin class switching in B lymphocytes. The half site (called a T-half site) is recognized by many other T-box proteins with different optimal target sequences that in part confer target gene specificity.