Promoter
EF1a.
Reporter
GFP.
Selection
Puromycin.
Species
Human/Mouse.
Serotype
VSV-G.
Application
Monitor transcriptional activity of NFκB p65 homodimer in mammalian cells, screen for activators or inhibitors of NFκB p65 homodimer signaling. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc. The REL-A (p65) reporter lentivirus contains tandem repeats of a homodimer p65 DNA-binding element that allows for the discrimination of p65/p65 homodimer vs p50/p65 heterodimer transcriptional activity when used alongside the pan NFκB reporter (LTV-0002). The NFκB p65 reporter could be useful for studying non-canonical NFκB signaling mechanisms such as in tumor cells as opposed to inflammation. This reporter differs from the pan NFκB reporter which can detect both p50/p65 heterodimers induced by TNFα, as well as p6/p65 homodimers.