Promoter
EF1a.
Reporter
GFP.
Selection
Blasticidin.
Species
Human/Mouse.
Serotype
VSV-G.
Application
Monitor NFE2 transcriptional activity in mammalian cells, screen for activators or inhibitors of NFE2 signaling. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc. NFE2 is essential for regulating erythroid and megakaryocytic maturation and differentiation, including platelet production, megakaryocyte biogenesis, and erythroid cell function. The NFE2 protein forms heterodimers with small MAF proteins such as MAF-G to regulate gene transcription. The NFE2 reporter lentivirus contains consensus NFE2 DNA-binding elements from the PBGD promoter to read out transcriptional activity of NFE2.