Promoter
EF1a.
Reporter
GFP.
Selection
Puromycin.
Species
Human/Mouse.
Serotype
VSV-G.
Application
Monitor transcriptional activity of MAFs (e.g. MAF-B, MAF-C, MAF-L) in mammalian cells, screen for activators or inhibitors of MAFs signaling. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc. MAFs contribute to the formation of lens cytoskeletal structure. Both MAF-C (c-MAF) and MAF-B are transcriptional activators, although MAF-B is less potent than MAF-C. MAF-C is a bona fide oncogene contributing to the progression of hematological malignancies and is overexpressed in other cancers, such as renal and head and neck cancer. MAF-B plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells, and is required for monocytic, macrophage, osteoclast, podocyte and islet beta cell differentiation. MAF-B also activates the insulin and glucagon promoters. The MARE reporter utilizes tandem repeats of T-MARE to read out transcriptional activity of MAFs in mammalian cells.