Promoter
EF1a.
Reporter
Renilla Luc.
Selection
Blasticidin.
Species
Human/Mouse.
Serotype
VSV-G.
Application
Monitor transcriptional activity of transcriptional factor E-B (TFEB) that acts as a master regulator of the CLEAR gene network to regulate genes related to autophagy, vesicular trafficking proteins, lysosomal enzymes, and lysosomal structural proteins. The tandem repeats of modified CLEAR elements enable one to detect activation of autophagy-lysosome signaling pathways activated by stressors, nutrient deprivation and inhibition of mTOR-AKT signaling in mammalian cells.