Promoter
EF1a.
Reporter
Renilla Luc.
Selection
Blasticidin.
Species
Human/Mouse.
Serotype
VSV-G.
Application
Monitor FOXO1 activity in mammalian cells, screen for activators or inhibitors of FOXO1. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc. Preferentially activated by FOXO1 (vs FOXO3). FOXO1-induced activation is inhibited by FOXP1. The IRE enables the reporter to be used to detect FOXO1 transcriptional inhibition via insulin/Akt pathway activation.