Promoter
CMV.
Reporter
hCDT1-RFP + hGemn-GFP.
Selection
Puromycin.
Species
Human/Mouse.
Serotype
VSV-G.
Application
A simple, fluorescence-based method to visualize cell cycle progression. The pre-packaged lentivirus is a convenient format that allows you to create stable cell lines easily within about 10 days after transducing your cells. Simply add the Fucci lentivirus to the cells and wait 2-3 days, then select with puromycin or blasticidin (depending on the product) for 2-3 days. After this, the cells can be visualized under fluorescence microscope and can be seeded to perform cell cycle assays based on your application.