Promoter
EF1a.
Reporter
GFP.
Selection
Puromycin.
Species
Human/Mouse.
Serotype
VSV-G.
Application
Monitor FOXN1 transcriptional activity in mammalian cells, screen for activators or inhibitors of FOXN1 signaling. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc. There are nearly 50 forkhead transcription factors (TFs) encoded in the human genome, all of which share a similar core DNA binding element, but differ in the flanking sequences that confer binding specificity for different forkhead TFs. The FOXN1 reporter utilizes forkhead TF response elements that have been optimized preferential binding of FOXN1 vs other forkhead TFs.