Promoter
EF1a.
Reporter
GFP.
Selection
Blasticidin.
Species
Human/Mouse.
Serotype
VSV-G.
Application
Monitor FOXM1 transcriptional activity in mammalian cells, screen for activators or inhibitors of FOXM1 signaling. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc. There are nearly 50 forkhead transcription factors (TFs) encoded in the human genome, all of which share a similar core DNA binding element, but differ in the flanking sequences that confer binding specificity for different forkhead TFs. The FOXM1 reporter utilizes forkhead TF response elements that have been optimized preferential binding of FOXM1 vs other forkhead TFs. FOXM1 is overexpressed in many cancers and is a key target for cancer therapy. The tandem repeats of FOXM1 DNA-binding elements enable to detect the FOXM activity and related cell cycle signaling cascade.