Promoter
EF1a.
Reporter
Firefly Luc.
Selection
Puromycin.
Species
Human/Mouse.
Serotype
VSV-G.
Application
Monitor transcriptional activity of bone morphogenic protein (BMP) signaling pathway in mammalian cells, screen for activators or inhibitors of BMP signaling pathway. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc. The BMP response element (BRE) reporter lentivirus is constructed with inverted repeats of mouse Id2 promoter BRE plus Smad4 DNA-binding motif to respond to BMP proteins. BMP signaling pathway is associated with embryogenesis, organ development, and adult tissue homeostasis. Smad1/5/8 are activated by BMP signal transduction, which form a complex with Smad4 that binds BRE sites in the promoters of target genes to regulate gene expression.